CRO Expertise

NEUROLOGICAL DOSING ROUTES (mice, rats, rabbits, NHPs)

• IT (Intrathecal)
• ICM (Intra Cisterna Magna)
• ICV (Intra Cerebral Ventricular)

CSF COLLECTION

INTRATHECAL PORTS FOR DRUG ADMINISTRATION
AND/OR CEREBROSPINAL FLUID (CSF) COLLECTION
IN NON-HUMAN PRIMATES (NHP)

OVERVIEW

Intrathecal administration (IT) is a route of administration for drugs via an injection into the spinal canal, or into the subarachnoid space so that it reaches the cerebrospinal fluid (CSF). Traditionally, the IT route of administration has been used to bypass the blood brain barrier (BBB).

Drugs may be administered by standard routes (IV, Oral) with CSF collection to assess CNS drug exposure and biomarker levels.

NHP MODEL

The anatomy and physiology of CNS models are comparable to a human.

CATHETERIZATION OF THE SPINAL IT SPACE

CSF catheters are implanted surgically via lumbar hemilaminectomy with catheter tip targeted for level of the cisterna magna. Access port is located on the back for ease of use.

INTRATHECAL (IT) INFUSION OF THERAPEUTIC AGENTS (TA)

IT lumbar dosing is utilized when:

• The TA cannot cross BBB and standard routes of drug therapy are not feasible
• TA must be delivered directly to the CNS to achieve efficacious doses

CSF COLLECTION

• Chronic ports allow for serial sampling of CSF in conscious NHP
• A properly working CSF port virtually eliminates contamination of CSF with red blood cells

OUR SUCCESS RATE

• Chronic ports allow for serial sampling of CSF in conscious NHP
• The Biomere colony allows for CSF ported models to be placed on study
• >95% success rate on sample collection
• Facility colony models have been patent for 2 years and counting

BIOMERE EXPERTISE

In addition to chronic porting, our expertise includes experience with direct stick to the lumbar and cisternal intrathecal space for a variety of both IT dosing and CSF collection study.

White Papers

Webinars

Molly Shoichet, University of Toronto – Regenerative Strategies in the Central Nervous System, Part One

Molly Shoichet, University of Toronto – Regenerative Strategies in the Central Nervous System, Part Two

Technical Sheets

CSF Collection in NHPs

Application Notes

CSF Sampling to Aid Diagnosis and Preclinical Drug Discovery

Articles

Functional mag resonance imaging awake transg fragile X rats

Identifying the integrated neural networks involved in capsaician-induced pain using fMRI in awake TRPV1 knockout and wild-type rats

The Serotonin Receptor 6 Antagonist Idalopirdine and Acetylcholinesterase Inhibitor Donepezil Have Synergistic Effects on Brain Activity A Functional MRI Study in the Awake Rat

Posters

Spinal Anesthesia in Infant Rats: Development of a Model, Preliminary Observations, and Assessment of Neurologic Outcomes

JOINN Locations