Laboratory Sciences

CRO Expertise

Our Laboratory Sciences team is positioned to support your study through multiple ex-vivo analyses. These include automated clinical pathology sample analysis through more complex flow cytometry analysis.

CLINICAL PATHOLOGY TISSUE CULTURE BIOMARKERS/BIOANALYSIS

LABORATORY SCIENCES
Biomere has extensive capabilities to support ex vivo or in vitro analytical needs. We will work with you to develop and qualify a variety of analytical methods, analyze and interpret flow cytometry data and complete clinical pathology in support of your in vivo studies.

BIOMERE EXPERTISE
Our team’s expertise with multiple models of disease in a variety of animal species works in parallel with their extensive experience aligning in vitro tools in support of in vivo studies.

CLINICAL PATHOLOGY

Our Clinical Pathology lab supports in vitro analyses of your in vivo studies through testing of blood and other bodily fluids, tissues, and microscopic evaluation of individual cells. Our highly-trained medical technologists are ready to support the needs of your studies.

Hematology and coagulation**
Clinical biochemistry (including microsampling (<10ul))
Urinalysis*
Bone marrow and bronchoalveolar sample staining/counts**
Platelet function
Cytology (CSF, BAL, etc.)
Hormones
Hemolytic studies
Assay/Method qualification

*Performed by subcontractor
**Coagulation and Stain Counts performed by subcontractor

TISSUE CULTURE

Our Tissue Culture lab supports in vitro analyses of your in vivo studies by performing tissue isolation, culturing of primary cells and cell lines for studies involving cell-based therapies, and performing cellular based assays.

Plaque Assay–Crystal Violet

Tissue/Cell Isolation and Culturing

Hepatocytes
Splenocytes
PBMCs
Tissue homogenization (GentleMACS Dissociator,GentleMACS Octo Dissociator with Heaters, MPBio FastPrep Homogenizer)
Magnetic bead isolation (Stemcell and Miltenyi)
Other cell isolations per request
Primary cell culture (including human PBMCs)
Cell line culture

Cellular Based Assays

Transfection assays
Cell viability, proliferation, and death assays
Fluorescent and luminescent cell-based assays

BIOMARKER AND BIOANALYTICAL

Our lab supports your in vivo work with a wide range of methods and platforms to analyze samples for key analytes and biomarkers. We look forward to partnering with you to customize the use of each platform to meet your research needs.

ELISAs

Commercially available ELISAs
Transfer of ELISA protocols in house

MSD

Single and multiplex cytokine/biomarker analysis
Custom panels

Flow Cytometry

Mouse, humanized mice, NHPs available – rat in progress
Multi color flow cytometry capabilities with a potential of up to 16 colors
Surface and intracellular markers
Receptor occupancy
Intracellular cytokine staining (ICS)
Cell stimulation
Cell sorting*
Data analysis

*3rd party contract

Example Flow Cytometry Gating Strategy

In this example gating strategy, we are able to identify the frequency of NK cells and B cells using multi-color flow cytometry. Our scientific team is ready to work with you to help build the optimal flow cytometry panel to meet your needs.