CRO Expertise
INFLAMMATORY BOWEL DISEASE (IBD)
Inflammation is a vital part of the body’s immune response. It is the body’s attempt to heal itself after an injury; defend itself against foreign invaders, such as viruses and bacteria; and repair damaged tissue. Biomere’s interest in inflammatory disease stems from our expertise in autoimmunity.
We offer models of systemic lupus erythematosus (SLE) as well as antigen induced infl ammation such as collagen induced arthritis and myelin peptide-induced experimental autoimmune encephalomyelitis (EAE) as a model of multiple sclerosis. Additionally we offer chemically induced models of infl ammatory bowel disease (IBD)/ulcerative colitis and Type 1 diabetes.
INDUCIBLE MODELS
Acute 2,4 6-trinitrobenzenesulfonic acid (TNBS) hapten including IFN-γ, TNF-α, IL-2, IL-12 and IL-18
- Mediated by Th1 and Th17 cells locally producing excessive proinflammatory cytokines
Acute dextran sulfate sodium (DSS)
- Suggested epithelial cell toxicity, increased intestinal permeability, and macrophage activation
IBD Readouts
IBD Disease Activity Index
- Body weight
- Rectal bleeding scoring (hemoccult kit)
- Stool scoring
Additional Analyses
- Interim blood collections for PK/PD evaluation
- Histology
- Collections for PK/PD evaluation
Colon Evaluation
- Colon length and weight measures
- Macroscopic colon scoring index
- 0 – 10 graded morphologic scoring
Microscopic Colon Scoring
- Histopathology assessment by an unbiased pathologist
- Inflammation and morphological damage
DSS Induced IBD in Mice
- 8 -10 animals per group
- Induction with 5% DSS in drinking water for consecutive days
- ~100% induction rate
- Disease onset on Day 3
- Peak disease on Day 8 – 10
- Positive control is available
TNBS-Induced IBD in Mice
- 8 -10 animals per group
- I~100% induction rate, low mortality
- Prophylactic or therapeutic treatment
- Intrarectal induction with TNBS hapten
- Study duration 5 to 8 days
– ~Day 5 is the peak of diseases severity
– Day 8 represents a recovery phase
